Structure of the O-Specific Polysaccharide ofHafnia Alvei23 Having an Oligosaccharide-Phosphate Repeating Unit
作者:
Ewa Katzenellenbogen,
Nina A. Kocharova,
Georgy V. Zatonsky,
Maria Bogulska,
Danuta Witkowska,
Aleksander S. Shashkov,
Yuriy A. Knirel,
Elzbieta Romanowska,
期刊:
Journal of Carbohydrate Chemistry
(Taylor Available online 1999)
卷期:
Volume 18,
issue 5
页码: 545-558
ISSN:0732-8303
年代: 1999
DOI:10.1080/07328309908544018
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Lipopolysaccharide (LPS) ofHafnia alvei23 has an acid-labile O-specific polysaccharide (OPS) with a pentasaccharide-phosphate repeating unit containing D-Glc1P, D-GlcNAc, L-Fuc, 6-deoxy-D-talose (D-6dTal), 4-acetamido-4,6-dideoxy-D-glucose (D-Qui4NAc), and anO-acetyl group. A partially degraded OPS was obtained by hydrolysis of LPS with 0.25 M sodium acetate in aqueous 0.5% acetic acid. Fractionation of LPS on Sephadex G-200 in DOC buffer allowed isolation of long-chain LPS species which, together with OPS, were studied by methylation analysis, chemical degradations (O-deacetylation, dephosphorylation with 48% hydrofluoric acid, Smith degradation), and1H and13C NMR spectroscopy, including 2D COSY, TOCSY, NOESY, and H-detected1H,13C heteronuclear single-quantum coherence (HSQC) experiments. The following structure of the repeating unit of OPS was established:
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