Fast Amperometric Immunoassay Utilizing Highly Dispersed Electrode Material
作者:
R. Krishnan,
A.L. Ghindilis,
P. Atanasov,
E. Wilkins,
期刊:
Analytical Letters
(Taylor Available online 1995)
卷期:
Volume 28,
issue 14
页码: 2459-2474
ISSN:0003-2719
年代: 1995
DOI:10.1080/00032719508004028
出版商: Taylor & Francis Group
关键词: amperometric immunoassay;immunoelectrode;immunosensor
数据来源: Taylor
摘要:
An immunoassay technique based on a high surface area carbon immunoelectrode is described. Dispersed ULTI carbon serves as a carrier for immobilized antibodies and also as an electrode material. ‘Sandwich’ scheme of immunoassay of rabbit IgG (as a model analyte) has been used. Iodine formed as a result of the enzymatic oxidation of iodide by peroxidase-label has been detected amperometrically. Using dispersed carbon material as a solid support for immobilization of immunoagents and as an electrode material improves the efficiency of immuno-interaction and the sensitivity of electrochemical assay due to the high area-to-volume ratio of solid to liquid phases.
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