ANALYSIS OF PHOSPHOENKEPHALINS BY COMBINED HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND ELECTROSPRAY IONIZATION MASS SPECTROMETRY
作者:
Xirong Zhu,
Chhabil Dass,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1999)
卷期:
Volume 22,
issue 11
页码: 1635-1647
ISSN:1082-6076
年代: 1999
DOI:10.1081/JLC-100101757
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
A method based upon a combination of high performance liquid chromatography (HPLC) and electrospray ionization (ESI) mass spectrometry (MS) for the analysis of phosphorylated peptides in a complex mixture of 14 phosphoenkephalins and their nonphosphorylated analogues is described. A narrow-bore column is directly connected to the ESI interface without the need of a solvent flow-splitter. A full-scan mass analysis in the positive-ion mode is used to provide the total ion current chromatogram and the molecular mass of each eluting component. Selective detection of phosphorylated peptides is achieved by selected-ion recording of the phosphate group marker ion (PO3−) in the negative-ion mode. These two scans are acquired simultaneously on a single injection of the sample. The influence of water clusters on background ion current is also discussed.
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