In vitro toxicological investigations of isoxazolinone amino acids ofLathyrus sativus
作者:
Matthias Riepe,
Peter S. Spencer,
Fernand Lambein,
Albert C. Ludolph,
Charles N. Allen,
期刊:
Natural Toxins
(WILEY Available online 1995)
卷期:
Volume 3,
issue 1
页码: 58-64
ISSN:1056-9014
年代: 1995
DOI:10.1002/nt.2620030111
出版商: John Wiley&Sons, Inc.
关键词: 3‐N‐oxalyl‐L‐2,3‐diaminopropanoic acid (β‐ODAP);β‐oxalylamino‐alanine (BOAA);α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid (AMPA);Excitotoxicity;Lathyrism;Hippocampal cultures
数据来源: WILEY
摘要:
AbstractTwo non‐protein amino acids ofLathyrus sativus, β‐(isoxazoline‐5‐on‐2‐yl)‐alanine (BIA) and its higher homologue α‐amino‐γ‐Cisoxazoline‐5‐on‐2‐yl)‐alanine (ACI) were tested for excitotoxic potential. BIA (0.5‐2.0 mM) but not ACI (2.0 mM) produced a concentration‐dependent neurodegeneration in mouse cortical explants. The neuronal damage was prevented by the prior and simultaneous application of 6‐cyano‐7‐nitroquinoxaline‐2,3‐dione (CNQX), indicating that it was mediated by non‐N‐methyl‐D‐aspartate type receptors. BIA (0,5‐2.0 mM) activated CNQX‐sensitive currents which were significantly smaller than those activated by 3‐N‐oxalyl‐L‐2,3‐diaminopropanoic acid (β‐ODAP) or α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazole‐propionic acid (AMPA) in the majority of neurons. In a small number of cells, BIA (2 mM) produced currents which were similar in amplitude to those activated by β‐ODAP (50 μM). These results suggest thatLathyrus sativusplants engineered to block the synthesis of β‐ODAP may accumulate a neurotoxic precurs
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