Abstract23, 24‐Dihydrocucurbitacin‐F has been isolated fromhemsleya amabilis. Its structure was studied in detail by1H‐ and13C‐,1H,1H COSY,1H,13C HETCOR,1H,13C LOCOR, and 1D NOE NMR spectroscopy. All1H‐ and13C‐spectral data for this compound have been assigned, and some assignments of1H and13C signals have been revised based on the 2D NMR spectra. In particular, previously unassigned methyl and hydroxy signals have been completely assigned via NOE and1H,13C‐LOCO