Cloning of an Inducible Nitric Oxide Synthase from Rat Polymorphonuclear Neutrophils
作者:
KosugaKunihiko,
YuiYoshiki,
HattoriRyuichi,
SaseKazuhiro,
EizawaHiroshi,
AoyamaTakeshi,
InoueReiko,
SasayamaShigetake,
期刊:
Endothelium
(Taylor Available online 1994)
卷期:
Volume 2,
issue 3
页码: 217-221
ISSN:1062-3329
年代: 1994
DOI:10.3109/10623329409053378
出版商: Taylor&Francis
关键词: nitric;oxide;synthase;neutrophil;cloning
数据来源: Taylor
摘要:
We have cloned a cDNA encoding rat neutrophil inducible nitric oxide synthase (iNOS). The amino acid sequence of the iNOS in the rat neutrophils was 95% identical to that in the mouse macrophages, and the difference between the two sequences was most likely the result of species variability. Moreover, the iNOS in the rat neutrophils was 99% identical in amino acid sequence to that in the rat hepatocytes and vascular smooth muscle cells (VSMC). Northern blot analysis of total RNA from both rat macrophages and neutrophils showed a single band above 4 kilo-nucleotides. These findings suggest that iNOS in rat neutrophils, macrophages, hepatocytes, and VSMC are almost identical in the primary structure.
点击下载:
PDF (419KB)
返 回