Chromosome Localization and Rflp Analysis of Pdc-E2: the Major Autoantigen of Primary Biliary Cirrhosis
作者:
LeungPatrick S. C.,
WatanabeYasuyuki,
MunozSantiago,
TeuberSuzanne S.,
PatelMulchand S.,
KorenbergJulie R.,
HaraPaul,
CoppelRoss,
GershwinM. Eric,
期刊:
Autoimmunity
(Taylor Available online 1993)
卷期:
Volume 14,
issue 4
页码: 335-340
ISSN:0891-6934
年代: 1993
DOI:10.3109/08916939309079237
出版商: Taylor&Francis
关键词: Primary biliary cirrhosis;PDC-E2;auto antigens
数据来源: Taylor
摘要:
Patients with primary biliary cirrhosis are well known for the presence of high titer antibodies against dihydrolipoamide acetyltransferase. the E2 subunit of the pyruvate dehydrogenase complex. We have taken advantage of a cDNA probe for dihydrolipoamide acetyltransferase to explore the possibility of polymorphism of the E2 subunit by probing genomic DNA from 38 patients with primary biliary cirrhosis and 26 healthy controls. To detect restriction fragment length polymorphism, DNA was digested with ten specific restriction enzymes that often detect polymorphism, including Barn HI, Bgl II. Eco RI, Hind III, Hinf I, Msp I, Pst I, Pvu II, Rsa I and Taq I. A Taq I polymorphism was found in 19 of 38 patients with PBC and 6 of 26 normal controls. In addition, using fluorescencein situhybridization, the gene for dihydrolipoamide acetyltransferase was mapped on human chromosome 11 band q23.1. Interestingly, this region of the long arm of chromosome 11 is often associated with cyto genetic abnormalities, including translocations.
点击下载:
PDF (913KB)
返 回