Isoelectric focusing of peroxidases released from soybean hypocotyl cell walls bySclerotium rolfsiiculture filtrate
作者:
C. R. Curtis,
Neal M. Barnett,
期刊:
Canadian Journal of Botany
(NRC Available online 1974)
卷期:
Volume 52,
issue 9
页码: 2037-2040
ISSN:0008-4026
年代: 1974
DOI:10.1139/b74-261
出版商: NRC Research Press
数据来源: NRC
摘要:
Bound soybean hypocotyl cell wall proteins can be released from the cell wall by incubation with a culture filtrate of the fungal pathogenSclerotium rolfsii. The released cell wall proteins were then separated by disc electrophoresis or isoelectric-focusing techniques in polyacrylamide gel columns and tested for peroxidase and esterase activity. Released cell wall proteins showed considerable peroxidase multiplicity, which was totally absent in the separation of fungal culture filtrate. Peroxidase patterns of some released cell wall peroxidases were similar to soybean cytoplasmic peroxidase patterns. In separate experiments, esterase bands were detected in the released cell wall proteins, but this was probably due to esterase activity of the fungal culture filtrate. Cytoplasmic esterases exhibited much higher isoelectric points than cell wall and culture filtrate esterases, which were concentrated between pH 3 and 6.
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