Potentiation of oxidative damage to rat red blood cells by the concurrent presence oft‐butyl hydroperoxide and bromotrichloromethane
作者:
Mitsuaki Sano,
Hideki Kawabata,
Isao Tomita,
Hisashi Yoshioka,
Miao‐Lin Hu,
期刊:
Journal of Toxicology and Environmental Health
(Taylor Available online 1994)
卷期:
Volume 43,
issue 3
页码: 339-350
ISSN:0098-4108
年代: 1994
DOI:10.1080/15287399409531925
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Recently potentiation of oxidative damage in rat red blood cells (rRBC) incubated with t‐butylhydroperoxide (BHP) in combination with bromotrichloromethane (BrCCI3) was demonstrated. The mechanism by which this combination (BrCCI3/BHP) potentiates the oxidative damage to rRBC was investigated in this study. When rRBC were incubated with 0.1 mM BHP, 0.5 mM BrCCI3, or the two combined, BrCCI3/BHP‐potentiated lipid peroxidation and hemolysis were further enhanced under anaerobic conditions. However, the potentiation of lipid peroxidation was abolished by heating or trypsin digestion of rRBC. Electron spin resonance (ESR) studies demonstrated an increase of alkoxyl radical induced by BrCCI3/BHP in rRBC, and this increase was abolished by heating or predigestion of hemolysates with trypsin. The inhibition of lipid peroxidation by diphenylamine (which reacts with alkoxyl radicals but not peroxyl radicals) suggests an important role of alkoxyl radicals. Overall, the present findings demonstrate that the increase in radical‐related oxidative damage, possibly mediated by proteinlike materials, may be at least partially responsible for the potentiation of damage to rRBC induced by BrCCI3/BHP, and perhaps by BrCCI3. Although the in vivo significance of these results remains to be investigated, it seems likely that halocarbon toxicity may be amplified by elevated levels of lipid peroxide in blood.
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