Identification of Proteins Using Affinity Affi-Gel Simultaneously with Acrylamide Electrophoresis
作者:
R.L. Bartzatt,
期刊:
Analytical Letters
(Taylor Available online 1995)
卷期:
Volume 28,
issue 15
页码: 2653-2661
ISSN:0003-2719
年代: 1995
DOI:10.1080/00032719508007416
出版商: Taylor & Francis Group
关键词: affinity;PAGE;electrophoresis
数据来源: Taylor
摘要:
This work presents a method in which a desired protein may be separated from a biological protein preparation and identified by highly specific monoclonal antibody. The desired protein may be isolated from crude protein preparations by this method. The protein remains intact and stable throughout the procedure. This method utilizes common nonreducing conditions of polyacrylamide gel electrophoresis (PAGE), and Western Blot transfer of protein. This isolation of the desired protein can be accomplished for a microgram or tens of micrograms of material. The application of Western blot technique with monoclonal antibody permits the highest specificity in terms of identifying the target protein. In addition to identifying the target protein the method can isolate sufficient amounts for further characterization.
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