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The Construction of an Amperometric Immunosensor for the Thyroid Hormone (+)-3,3′,5-Triiodo-L-Thyronine (L-T3)

 

作者: Hassan Y. Aboul-Enein,   Raluca-Ioana Stefan,   Gabriel Lucian Radu,   George-Emil Baiulescu,  

 

期刊: Analytical Letters  （Taylor Available online 1999）
卷期: Volume 32, issue 3  

页码: 447-455

 

ISSN:0003-2719

 

年代: 1999

 

DOI:10.1080/00032719908542832

 

出版商: Taylor & Francis Group

 

关键词: Amperometric immunosensor;Graphite paste;Thyroid hormone;(+)-3,3′,5-triiodo-L-thyronine;Mouse monoclonal anti-(+)-3,3′,5-triiodo-L-thyronine.

 

数据来源: Taylor

 

摘要:

A novel amperometric immunosensor based on graphite paste (graphite powder and paraffin oil) has been constructed. The graphite paste is impregnated with mouse monoclonal anti-(+)-3,3′,5-triiodo-L-thyronine (anti-T3). The immunosensor can be reliably used for L-T3assay in thyroid and in drugs, using chronoamperometric technique, at ppb-ppt concentration levels. The potential used for L-T3assay was 650 mV vs Ag/AgCl. The surface of the immunosensor can be regenerated by simply polishing, obtaining fresh immunocomposite ready to be used in a new assay.

 

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