Enantiomeric Separation of Non-Protein Amino Acids by Chiral Ligand-Exchange High-Performance Liquid Chromatography
作者:
Toshifumi Miyazawa,
Hiroc Minowa,
Kiwamu Imagawa,
Takashi Yamada,
期刊:
Analytical Letters
(Taylor Available online 1997)
卷期:
Volume 30,
issue 4
页码: 867-882
ISSN:0003-2719
年代: 1997
DOI:10.1080/00032719708006430
出版商: Taylor & Francis Group
关键词: enantiomeric separation;non-protein amino acids;high-performance liquid chromatography;chiral ligand-exchange phase
数据来源: Taylor
摘要:
The enantiomeric separation by high-performance liquid chromatography of underivatized non-protein amino acids was investigated by using a column packed with octadecylsilanized silica coated withN,S-dioctyl-D-penicillamine as a chiral ligand-exchange phase (Sumichiral OA-5000). Excellent to good separations of enantiomers were achieved with a variety of nonprotein amino acids carrying aliphatic or aromatic side-chains by optimizing the amount (0-20%, v/v) of the organic component (2-propanol) and the concentration (1-5 mM) of the complexing metal ion (Cu2+) in the hydro-organic eluent.
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