Ultrastructural localization of hydrogen peroxide in experimental autoimmune uveitis
作者:
ShuangGuey,
GritzDavid C.,
AtallaLily R.,
StanforthDavid A.,
SevanianAlex,
RaoNarsing A.,
期刊:
Current Eye Research
(Taylor Available online 1992)
卷期:
Volume 11,
issue 10
页码: 955-961
ISSN:0271-3683
年代: 1992
DOI:10.3109/02713689209033493
出版商: Taylor&Francis
数据来源: Taylor
摘要:
One of the most prominent features of S-antigen induced uveitis is the massive infiltration of polymorphonuclear leukocytes (PMNs) and mononuclear cells in the ocular tissues and fluids. These inflammatory cells generate reactive oxygen metabolites as microbicidal agents and release these oxidants into the surrounding tissues. Using the cerium perhydroxide method, we have localized subcellular hydrogen peroxide in various inflamed ocular tissues. Most notably, the positive electron-dense granules were seen in the plasma membranes of PMNs that were infiltrating in the retina and uvea. These deposits were noted also in PMNs located within the extravascular spaces. For the intravascular PMNs, the positive reaction products were seen in much lower concentrations. A direct demonstration of substantial concentrations of hydrogen peroxide in experimental autoimmune uveitis, therefore, suggests the possibility that this reactive metabolite is an inflammatory mediator in this condition.
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