A Comparison of Different Stains Used for the Detection of Aspartate Akinotsansferase in Biological Samples
作者:
C. Quiroga,
S. Imperial,
M. Busquets,
A. Cortés,
J. Bozal,
期刊:
Analytical Letters
(Taylor Available online 1987)
卷期:
Volume 20,
issue 4
页码: 569-585
ISSN:0003-2719
年代: 1987
DOI:10.1080/00032718708067990
出版商: Taylor & Francis Group
关键词: Aspartate aminotransferase;disc electrophoresis;stains;human;rat;chicken.
数据来源: Taylor
摘要:
Four staining methods for aspartate aminotransferase (AAT) detection after electrophoresis have been reported. Comparison of the minimum AAT activity detected by these methods showed the following sequence: Sakakibara et al.> Rej and Herder > Decker and Rau > Banks et al. Addition of ADP and diaphorase to the stain of Banks increased its sensitivity 10 fold. The presence of lactate dehydrogenase (LDH > activity in the samples gave electrophoretical artifacts when the methods of Banks et al. and Rej & HoSrder were used. These artifacts were due to the nothing dehydrogenase (NoDH) activity of LDH as demonstrated by chromatography on 5′ AMP-Sepharose 4B (LDH affinity support).
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