A fluorescent artificial receptor previously synthesized in our group, is shown to be able to bind metal ions after dimerization. The monomer is a urea macrocyclophane: (13,21-Bis(methoxycarbonyl)-1,9,17-trioxo-2,8,16,18-tetraaza[9.3]metaorthocyclophane. The dimer is used to quantify Zn(II) through the fluorescence signal change observed upon complexation. This change can be related either to the decrease of the monomer or to the increase of the dimer forms. The response is linear for a Zn(II) concentration range between 5 and 50 μM. Specificity for Zn(II), examplified by comparison with Cd(II). was found acceptable.