Quantification and Distribution of Troponin-T in Cultures of Chick Embryo Myocardiocytes
作者:
G. Vélez,
A.E. Aránega,
M.A. Muros,
F.J. González,
J.C. Prados-Salazar,
L. Alvarez,
A. Aránega,
期刊:
Cells Tissues Organs
(Karger Available online 1992)
卷期:
Volume 145,
issue 3
页码: 269-276
ISSN:1422-6405
年代: 1992
DOI:10.1159/000147376
出版商: S. Karger AG
关键词: Chick Culture;Myocardiocyte;Troponin-T
数据来源: Karger
摘要:
We analyzed the distribution and labeling patterns of troponin-T, a protein involved in the regulation of striated muscle contraction, in myocardiocytes obtained from chick embryos in Hamburger and Hamilton’s stage 25 and 39, and cultured for 8 days. Troponin-T expression was examined with indirect immunofluorescence, densitometry, fluorescence-activated cell sorting, electrophoresis and immunoblotting. The patterns of expression of troponin-T were compared with those of actin to determine possible correlations in different stages of chick embryo development and culture. Our findings show that in both stages of embryonic development, the cellular accumulation of troponin-T changed after 8 days of culture. Our results revealed a quantitative modification with time: after 4 days of culture there was a significant increase in this protein, followed by a slight additional increase after 8 days of culture. Flow cytometry findings confirmed these trends over time, showing a significant increase in positive cells after 4 days, followed by a smaller rise after 8 days of culture. In comparison with actin, this pattern was similar only in cells from Hamburger and Hamilton’s stage 25 embr
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