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Complementary Deoxyribonucleic Acid Cloning of an mRNA Encoding Transforming Growth Factor-β2 from Chicken Embryo Chondrocytes

 

作者: JakowlewSonia B.,   DillardPamela J.,   SpornMichael B.,   RobertsAnita B.,  

 

期刊: Growth Factors  (Taylor Available online 1990)
卷期: Volume 2, issue 2  

页码: 123-133

 

ISSN:0897-7194

 

年代: 1990

 

DOI:10.3109/08977199009071499

 

出版商: Taylor&Francis

 

关键词: TGF-β2 mRNA;cDNA cloning;chicken chondrocytes

 

数据来源: Taylor

 

摘要:

AbstractUsing a simian transforming growth factor-β2 (TGF-β2) cDNA probe, we have isolated chicken cDNA clones for TGF-β2 from a chicken embryo chondrocyte cDNA library. The predicted precursor protein of chicken TGF-β2 is 412 amino acids long, two amino acids shorter than the human form to which it shows 90% identity. Cleavage of the chicken TGF-β2 precursor at a pentabasic Arg-Arg-Lys-Lys-Arg site would produce a 112-amino acid processed peptide differing by only one amino acid from the human TGF-β2 peptide. In contrast to TGF-β3 and 4 mRNAs, TGF-β2 mRNA is expressed in both cultured and non-cultured chicken embryo chondrocytes and at higher levels in chicken embryo fibroblasts. In chondrocytes and fibroblasts, the 3.9- and 4.3-kb TGF-β2 mRNAs are both expressed at higher levels than the 8-kb TGF-β2 mRNA; however, in developing chicken embryos, the level of expression of the 8-kb mRNA is higher than that of the 3.9- and 4.3-kb mRNAs.

 

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