Complementary Deoxyribonucleic Acid Cloning of an mRNA Encoding Transforming Growth Factor-β2 from Chicken Embryo Chondrocytes
作者:
JakowlewSonia B.,
DillardPamela J.,
SpornMichael B.,
RobertsAnita B.,
期刊:
Growth Factors
(Taylor Available online 1990)
卷期:
Volume 2,
issue 2
页码: 123-133
ISSN:0897-7194
年代: 1990
DOI:10.3109/08977199009071499
出版商: Taylor&Francis
关键词: TGF-β2 mRNA;cDNA cloning;chicken chondrocytes
数据来源: Taylor
摘要:
AbstractUsing a simian transforming growth factor-β2 (TGF-β2) cDNA probe, we have isolated chicken cDNA clones for TGF-β2 from a chicken embryo chondrocyte cDNA library. The predicted precursor protein of chicken TGF-β2 is 412 amino acids long, two amino acids shorter than the human form to which it shows 90% identity. Cleavage of the chicken TGF-β2 precursor at a pentabasic Arg-Arg-Lys-Lys-Arg site would produce a 112-amino acid processed peptide differing by only one amino acid from the human TGF-β2 peptide. In contrast to TGF-β3 and 4 mRNAs, TGF-β2 mRNA is expressed in both cultured and non-cultured chicken embryo chondrocytes and at higher levels in chicken embryo fibroblasts. In chondrocytes and fibroblasts, the 3.9- and 4.3-kb TGF-β2 mRNAs are both expressed at higher levels than the 8-kb TGF-β2 mRNA; however, in developing chicken embryos, the level of expression of the 8-kb mRNA is higher than that of the 3.9- and 4.3-kb mRNAs.
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