α2-HS-glycoprotein (AHSG) phenotypes were determinated in 506 unrelated autochthonous individuals from the Galician population (NW Spain) by means of isoelectric focusing followed by silver stain immunofixation. The phenotype frequency distribution fitted with the Hardy-Weinberg law. No evidence of intrapopulation heterogeneity was observed. The allele frequencies obtained were: AHSG* 1 = 0.7559 ± 0.013 and AHSG*2 = 0.2441 ± 0.013. Our findings lend support to the hypothesis of an AHSG gene frequency cline in European populations. Other population genetics’ considerations have also been discu