Preparation of immobilized phosphodiesterase from calf spleen and its use in oligonucleotide analysis
作者:
H. Seliger,
E. H. Teufel,
M. Philipp,
期刊:
Biotechnology and Bioengineering
(WILEY Available online 1980)
卷期:
Volume 22,
issue 1
页码: 55-64
ISSN:0006-3592
年代: 1980
DOI:10.1002/bit.260220105
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractThe phosphodiesterase from calf spleen (EC 3.1.4.18) was immobilized on several supports. Some properties of the most suitable enzyme support system—calf spleen phosphodiesterase bound to agarose‐Concanavalin A—were investigated, e.g., pH dependence, influence of ionic strength of the buffer medium, and Zn2+‐ion inhibition. The immobilized spleen phosphodiesterase showed about 60% of the activity of the free enzyme: the activity toward several oligonucleotide test substrates was unchanged for two
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