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Microdetermination of Bile Acids by High-Performance Liquid Chromatography Equipped with a High Sensitive Conductance Detector

 

作者: Yohei Hashimoto,   Yoko Asai,   Masataka Moriyasu,   Akira Uji,  

 

期刊: Analytical Letters  （Taylor Available online 1981）
卷期: Volume 14, issue 17-18  

页码: 1483-1492

 

ISSN:0003-2719

 

年代: 1981

 

DOI:10.1080/00032718108081474

 

出版商: Taylor & Francis Group

 

关键词: high-performance liquid chromatography;bile acids;conductance detector

 

数据来源: Taylor

 

摘要:

Conductimetric detection of bile acids in reversed phase high-performance liquid chromatography is described. The solvent system of the mixture of water and organic solvent containing small amount of basic salts such as ammonium carbonate is found useable by removing the cation with the cation exchange column inserted between the ODS column and the conductance detector. Thus, a few ng of tauro-and glyco-conjugated bile acids can be detected without tedious derivatization and hydrolysis.

 

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