Determination of [13C-15N] Labeled Methylxanthines by Gas Chromatography-Atomic Emission Detection (GC-AED) and Compound-Independent Calibration
作者:
W. Elbast,
M.S. Caubet,
D. Deruaz,
J.L. Brazier,
期刊:
Analytical Letters
(Taylor Available online 1999)
卷期:
Volume 32,
issue 15
页码: 3111-3130
ISSN:0003-2719
年代: 1999
DOI:10.1080/00032719908543030
出版商: Taylor & Francis Group
关键词: Atomic Emission Detection;Compound-Independent Calibration - CIC;[13C-15N] Labeled methylxanthines- Stable isotopes
数据来源: Taylor
摘要:
Compound independent calibration (CIC) can be used to measure stable isotopically labeled molecules and their metabolites. This study was designed to demonstrate that gas chromatography coupled to atomic emission detection and CIC are useful analytical tools for the specific and quantitative determination of13C and15N labeled caffeine and its metabolites. These labeled compounds can be measured by monitoring either13C or15N atomic emission lines and using only one calibration function from one of the analytes. This method can be helpful when it is difficult or impossible to obtain an authentic reference material, which is often the case in metabolic studies.
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