Identification of a Secretory IgA Receptor on Breast‐Milk MacrophagesEvidence for Specific Activation Via These Receptors
作者:
G. ROBINSON,
B. VOLOVITZ,
J. PASSWELL,
期刊:
Pediatric Research
(OVID Available online 1991)
卷期:
Volume 29,
issue 5
页码: 429-434
ISSN:0031-3998
年代: 1991
出版商: OVID
数据来源: OVID
摘要:
Binding of secretory IgA (sIgA) to human milk macrophages was identified by rosette formation with sIgA-sensitized indicator cells and competitive inhibition binding studies with [125I]-sIgA. Macrophages formed rosettes with sIgA-sensitized sheep red blood cells that were inhibited by sIgA (87%) but not IgG. Both IgA1and IgA2inhibited sIgA-sensitized sheep red blood cell rosette formation. Rosette formation was completely inhibited by galactose, partially inhibited by asialofetuin, and unaffected by mannose. [125I]-labeled sIgA binding to macrophages reached a plateau after 60 min, was dependent on the number of macrophages in culture, and was specifically inhibited by unlabeled sIgA. Incubation of macrophage monolayers with increasing concentrations of sIgA-anti-IgA immune complexes resulted in a progressive increase in the oxidative burst and increased secretion of prostaglandins. These studies indicate that human milk macrophages have a receptor for sIgA and that activation of these macrophages may occur via these receptors.
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