Measurement of cellulase and xylosidase activities in peat using a sensitive fluorogenic compound assay
作者:
H. Kang,
C. Freeman,
期刊:
Communications in Soil Science and Plant Analysis
(Taylor Available online 1998)
卷期:
Volume 29,
issue 17-18
页码: 2769-2774
ISSN:0010-3624
年代: 1998
DOI:10.1080/00103629809370151
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
We applied fluorogenic methylumbelliferyl compounds to measure enzyme activities (ß‐glucosidase, cellobiohydrolase, and ß‐xylosidase) in a wetland peat. The enzyme activities showed substrate saturation around 400 μM and approximate linearity for at least 100 minutes incubation time. The effects of phenolics and toluene on the enzymes were small. All enzyme activities increased with increasing incubation temperature. Beta‐glucosidase and ß‐xylosidase showed optimal activities in an acidic condition (pH 5), while cellobiohydrolase had the highest activity in a neutral pH. The method would be effective in samples with low enzyme activities and interferences from organic compounds.
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