High temperature disinfestation was investigated as an alternative, non-chemical treatment for the postharvest quarantine control of two-spotted mites (TSM,Tetranychus urticaeKoch) on nectarines. Diapausing TSM pre-conditioned for 28–30 days at 15°C, were killed by exposure to wann air at 46.7°C for 15.5 h, or 43.9°C for 34 h. When pre-conditioned at 4.0°C for 5–15 days complete mortality was observed after 28 h wann air at 48.2°C. These times and conditions were longer than those predicted to kill non-diapausing TSM when 100% mortality was observed at 24 h at air temperatures between 43.2 and 46.0°C.