Inhibition of Phytohaemagglutinin-Induced Lymphocyte Proliferan by Immunosuppressive Drugs: Use of Whole Blood Culture
作者:
PiekoszewskiWojciech,
ChowFung Sing,
JuskoWilliam J,
期刊:
Immunopharmacology and Immunotoxicology
(Taylor Available online 1994)
卷期:
Volume 16,
issue 3
页码: 389-401
ISSN:0892-3973
年代: 1994
DOI:10.3109/08923979409007100
出版商: Taylor&Francis
数据来源: Taylor
摘要:
AbstractA whole blood lymphocyte proliferation assay was compared to a standard method requiring the isolation of lymphocytes from blood. Both methods were used to measure inhibition of proliferative responses of phytohaemagglutinin (PHA1)-stimulated human peripheral blood lymphocytes by tacrolimus (FK 5061), cyclosporine A (CsA1), rapamycin (RA1), dexamethasone (DEX1), prednisolone (PR1), and methylprednisolone (MP1). Three of the drugs studied (FK 506, CsA, and DEX) yielded similar IC50values with both methods. The whole blood proliferation assay produced modestly lower IC50values for RA, PR and MP. The whole blood lymphocyte proliferation method is simple and can be used when only limited volumes of blood can be obtained or isolation of cells gives unsatisfactory yields.
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