Rapid Detection of Waterborne Viruses Using the Polymerase Chain Reaction and a Gene Probe
作者:
N. Jothikumar,
P. Khanna,
S. Kamatchiammal,
Paul Murugan,
期刊:
Intervirology
(Karger Available online 1992)
卷期:
Volume 34,
issue 4
页码: 184-191
ISSN:0300-5526
年代: 1992
DOI:10.1159/000150281
出版商: S. Karger AG
关键词: Waterborne viruses;Poliovirus 1;Virus concentration;Detection;Gene amplification;Gene probe
数据来源: Karger
摘要:
We describe a membrane-filter-based urea-arginine phosphate buffer method for concentrating waterborne viruses from large volumes of water to microlitre volumes, and their subsequent detection by the polymerase chain reaction (PCR). The detection step involves the extraction of RNA, synthesis of complementary DNA, amplification by PCR of target DNA with specific primers, and confirmation through nucleic acid hybridization with a radiolabelled oligonucleotide probe. The PCR technique detected the presence of enteroviruses in spiked as well as in contaminated water samples. The technique is sensitive and detects as few as 120 waterborne viral particles. PCR is simple, rapid, sensitive, specific and adaptable for water quality surveillance in less developed countries.
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