The male accessory reproductive glands (MARG) play an important role in contributing proteins for the formation of the spermatophore, a structure that transports sperm from the male to the female. Several studies have shown that these proteins could be either of intraglandular or extraglandular origin. SDS-PAGE analysis revealed proteins in the adult MARG ofChilo partellus, which comigrate with the larval hemolymph proteins (LHP). Ouchterlony's immunodiffusion showed that the antiserum raised against MARG extract cross-reacts with the proteins present in the adult hemolymph and fat body. Immunoprecipitates of labelled hemolymph proteins with anti-LHP or anti-MARG proteins reveal two polypeptides that comigrate with LHPs. Thus, there appear to be some proteins of extraglandular origin in the MARG ofC. partellus, and the LHP could belong to this category.