Effects of Elastase on Cultured Human Trabecular Meshwork
作者:
Takuo Hoya,
期刊:
Ophthalmologica
(Karger Available online 1992)
卷期:
Volume 204,
issue 3
页码: 156-161
ISSN:0030-3755
年代: 1992
DOI:10.1159/000310285
出版商: S. Karger AG
关键词: Trabecular meshwork;Human;Elastase;Extracellular materials;Electron microscopy;Morphometry
数据来源: Karger
摘要:
We evaluated the effect of elastase, a proteolytic enzyme, on the endothelial meshwork of cultured human trabecular meshwork using a solid agar method. The tissues were obtained from 3 postmortem eyes, aged 64, 71 and 77 years. Elastase was added to the culture medium for 24 h at a concentration of 1 × 10––1––10––4mg/ml. The cultured specimens were then processed for transmission electron microscopy. We evaluated the electron micrographs to determine the percent area occupied by the cells and the extracellular materials and by the empty space in the endothelial meshwork using computer-assisted morphometry. A statistically significant decrease in extracellular materials with intact trabecular cells was confirmed 24 h following the administration of elastase at the concentration of 1 × 10––1––10––3 mg/ml.
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