Use of computerized motility analyzer for the evaluation of frozen‐thawed ram spermatozoa
作者:
D. F. Moses,
M. A. Heras,
A. Valcárcel,
L. Pérez,
H. Baldassarre,
期刊:
Andrologia
(WILEY Available online 1995)
卷期:
Volume 27,
issue 1
页码: 25-29
ISSN:0303-4569
年代: 1995
DOI:10.1111/j.1439-0272.1995.tb02091.x
出版商: Blackwell Publishing Ltd
关键词: Key wordsSemen;cryopreservation;kinetic parameters;ram
数据来源: WILEY
摘要:
SummaryA computerized motility analyzer (CellTrak/STM) was calibrated for its use with ram semen. Adjustment of the several setup variables allowed an accurate measurement of kinetic parameters such as percentage of motile cells, straight velocity, curvilinear velocity, linearity and amplitude of lateral displacement of the head. All kinetic parameters, except the lateral displacement of the head, showed significant changes after freezing and thawing. The curvilinear velocity exhibited the least significative post‐thaw decrease. The alterations in kinetic parameters provoked by freezing and thawing could account for the low success obtained with frozen semen by cervical insemination, as it is accepted that during the initial steps of fertilization high motility and linearity are require
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