Bioluminescent Determination of ATP in Ischemic Rat Heart
作者:
R. Lindbrg,
T. Lövgren,
期刊:
Analytical Letters
(Taylor Available online 1982)
卷期:
Volume 15,
issue 2
页码: 115-121
ISSN:0003-2719
年代: 1982
DOI:10.1080/00032718208064369
出版商: Taylor & Francis Group
关键词: Bioluminescences;ATP;ischemie;heart
数据来源: Taylor
摘要:
Two different ATP extraction methods, one using perchloirc acid and the other boiling Tris bufer, for ATP determinations in myocardial samples werer investigated and compared. A bioluminescent assay was used to quantify the extracted ATP. In spite of some drawbacks, eprchloric acid extraction is zix times more efficient thatn boiling Tris buffer extraction for releasing ATP from myocardial tissue. The perchloric acid extraction was used to determine ATP depletion in the endocardium of isolated ischemic rat hearts.
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