首页   按字顺浏览 期刊浏览 卷期浏览 Screening method for the determination of peroxide acumulation in honey and relation wi...
Screening method for the determination of peroxide acumulation in honey and relation with HMF content

 

作者: KerkvlietJ D,  

 

期刊: Journal of Apicultural Research  (Taylor Available online 1996)
卷期: Volume 35, issue 3-4  

页码: 110-117

 

ISSN:0021-8839

 

年代: 1996

 

DOI:10.1080/00218839.1996.11100920

 

出版商: Taylor&Francis

 

关键词: honey;hydrogen peroxide;HMF;enzyme activity;glucose oxidase;catalase;diastase;heating;ascorbic acid;vitamin C

 

数据来源: Taylor

 

摘要:

SUMMARYA fast semiquantitative screening procedure is described for the determination of peroxide activity in honey. Upon dilution of honey with water, hydrogen peroxide is released by the action of the enzyme glucose oxidase. After 1 h incubation time at 20°C, one peroxide test strip (Merck) is dipped into the honey solution for one second and the blue colour obtained is read after 15 s by means of the colour scale. The obtained value, multiplied by five, gives the amount of hydrogen peroxide in micrograms per g honey per hour at 20°C. A zero value may result from heated honey or prolonged storage, by low natural enzyme content, by chemical interactions or by the action of the enzyme catalase. Vitamin C (ascorbic acid) also reduces hydrogen peroxide formation. After analysing about 500 honey samples, it was found that if the peroxide accumulation was≥10μg/g/h (20°C) HMF was≤40 mg/kg and/or the diastase index was≥8 with a probability of 95%.

 

点击下载:  PDF (403KB)



返 回