Improved techniques for the preparation of bacterial lipopolysaccharides
作者:
K. G. Johnson,
M. B. Perry,
期刊:
Canadian Journal of Microbiology
(NRC Available online 1976)
卷期:
Volume 22,
issue 1
页码: 29-34
ISSN:0008-4166
年代: 1976
DOI:10.1139/m76-004
出版商: NRC Research Press
数据来源: NRC
摘要:
Lipopolysaccharides were prepared from six organisms by the use of two cell-disruption procedures before conventional phenol–water extraction. Disruption of cells by grinding with glass beads or by digestion with hen egg white lysozyme before phenol extraction facilitated rapid purification and greater yields of lipopolysaccharide. Pretreatment of cells with lysozyme in the presence of ethylenediaminetetraacetic acid was the most efficient method in terms of lipopolysaccharide yield and ease of preparation. Increase in lipopolysaccharide yield achieved by use of the lysozyme method, compared with the conventional phenol extraction, varied from 1.7- to 12.4-fold. Preparations were designated as pure according to several criteria and were judged not to have undergone changes as a result of prephenol extraction procedures.
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