Opioid-Noradrenergic Interactions in the Neurohypophysis
作者:
Bai-ge Zhao,
Christopher Chapman,
John Bicknell,
期刊:
Neuroendocrinology
(Karger Available online 1988)
卷期:
Volume 48,
issue 1
页码: 16-24
ISSN:0028-3835
年代: 1988
DOI:10.1159/000124984
出版商: S. Karger AG
关键词: Opioid receptors;Oxytocin;Vasopressin;Noradrenaline;Neurohypophysis
数据来源: Karger
摘要:
The actions of opioids on electrically evoked release of oxytocin, vasopressin, and noradrenaline – using the [3H]-noradrenaline technique – from the rat neurohypophysis were examined in vitro. Antagonism of the action of endogenous neurohypophysial opioids with naloxone enhanced release of peptides and [3H]-noradrenaline differentially. Naloxone enhanced oxytocin release by 100 and 173% in two series of experiments (ED50 7× 10–7M), whilst vasopressin release was enhanced by only 30 and 20%, respectively. [3H]-noradrenaline release was maximally enhanced by 41% (ED50 2× 10–7M). We examined the opioid receptor subtypes mediating these effects using selective receptor agonists. The ĸ-agonist U-50,488H inhibited oxytocin and vasopressin release to a similar extent, but did not modify [3H]-noradrenaline release. The effects of U-50,488H were completely prevented by a tenfold molar excess of naloxone. The µ-agonist (Z)-Ala2, MePhe5 Gly-ol)-enkephalin also failed to inhibit [3H]-noradrenaline release and caused only a minor inhibition of oxytocin and vasopressin secretion. The δ-agonist (D-Pen2, D-Pen5)-enkephalin was without effect. We conclude that (1) ĸ-re-ceptors sensitive to U-50,488H mediate opioid inhibition of secretion from oxytocin and vasopressin nerve terminals; (2) when opioid actions are blocked by naloxone, opioid peptides within the neurohypophysis are shown to exert a much greater influence over oxytocin compared to vasopressin terminals; (3) neurohypophysial opioids also regulate release from noradrenergic terminals, although the nature of the receptors involved remains unclear, and (4) ĸ-receptors can mediate inhibition of neurohormone secretion by an action independent of the neurohypophysial noradrenerg
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