Three dimensional image reconstruction in conventional and confocal microscopy
作者:
T Wilson,
J B Tan,
期刊:
Bioimaging
(WILEY Available online 1993)
卷期:
Volume 1,
issue 3
页码: 176-184
ISSN:0966-9051
年代: 1993
DOI:10.1002/1361-6374(199309)1:3<176::AID-BIO6>3.0.CO;2-Y
出版商: IOP Publishing Ltd
关键词: confocal microscopy;3‐D optical sectioning image reconstruction
数据来源: WILEY
摘要:
AbstractWe discuss the consequences of implementing image reconstruction techniques which essentially aim to normalize the three dimensional transfer function to unity. We consider the fluorescence case in detail for confocal and conventional systems and show that a good deal of care needs to be taken in interpreting the result when images from conventional microscopes are processed. Brightfield confocal imaging is also considered.
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