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Rapid and Sensitive Spectrophotometry. III. A Double Beam Apparatus

 

作者: Britton Chance,  

 

期刊: Review of Scientific Instruments  (AIP Available online 1951)
卷期: Volume 22, issue 8  

页码: 634-638

 

ISSN:0034-6748

 

年代: 1951

 

DOI:10.1063/1.1746021

 

出版商: AIP

 

数据来源: AIP

 

摘要:

An apparatus for accurately timing the sequence of spectroscopic events in turbid suspensions of the cytochrome oxidase system has been developed. This apparatus measures simultaneously small optical density changes occurring at two wavelengths in the visible region with a small spectral interval in spite of the large scattering of light by the heart muscle suspensions. The cross talk between the two channels corresponds to an optical density change of less than 5×10−4. Transients arising from the modulation and demodulation processes cause no difficulty. The error of optical measurements caused by the noise level in the photocurrent is 2×10−4for a rise time (10–90 percent) of 1 sec. Some typical experimental results are included.

 

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