Quantitative High Performance Liquid Chromatographic Determination of Bamipine Combined with Tricyclic Antidepressants and/or Antipsychotics in Pharmaceutical Formulations
作者:
JohnE. Kountourellis,
CatherineK. Markopoulou,
JohnA. Stratis,
期刊:
Analytical Letters
(Taylor Available online 1993)
卷期:
Volume 26,
issue 10
页码: 2171-2182
ISSN:0003-2719
年代: 1993
DOI:10.1080/00032719308017460
出版商: Taylor & Francis Group
关键词: bamipine;chlorprothixene;clomipramine;haloperidol;prochlorperazine;trifluoperazine;thioridazine;trimipramine
数据来源: Taylor
摘要:
An advanced reserved phase High Performance Liquid Chromatographic (HPLC) method using UV detection, at 251, is presented for the simultaneous determination of bamipine combined with tricyclic antidepressants and/or antipsychotics in various pharmaceutical formulations. Sample analyses were performed on a bonded reserved-phase C-2,5 μm, 250 × 4.6 mm ID (Lichrosorb RP-2)column using aqueous ammonium acetate at constant ionic strength 0.015 M and acetonitrile 35: 65 as eluent, at a flow rate of 0.9 ml/min. The pH was adjusted to 4.7 with acetic acid. The presence of acetate buffer both shortens the elution time and improves the symmetry of the chromatographic peaks. The retention time was for bamipine, prochlorperazine, trifluoperazine, thioridazine, chlorprothixene, haloperidol, clomipramine, trimipramine 8.83, 11.83, 11.50, 11.08, 10.42, 8.05, 10.25, 9.67 min respectively. Linearity and precision data have been assessed. The method also involves an investigation of the dependence of the capacity ratio k′ on the pH and the polarity of the mobile phase.
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