Use of the Nonradioactive Iodine Label and the Avidinbiotin System for Immunoassays
作者:
M. Butler,
R. O'Kennedy,
期刊:
Analytical Letters
(Taylor Available online 1996)
卷期:
Volume 29,
issue 4
页码: 529-545
ISSN:0003-2719
年代: 1996
DOI:10.1080/00032719608000419
出版商: Taylor & Francis Group
关键词: non-radioactive iodine label;avidin;biotin;immunoassay
数据来源: Taylor
摘要:
The use of iodinated Bolton-Hunter reagent (IBHR) - labelled avidin and of IBHR-labelled biotin in immunoassays for the measurement of human IgG is investigated. The IBHR label is detected using the reaction between cerium(IV) and antimony(III). The results are compared to those obtained for immunoassays using IBHR-labelled antibodies or enzyme-labelled antibodies.
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