pH Changes in Immobiline gels due to low‐molecular mass ion adsorption and condition for salt front formation during electrophoretic desorption
作者:
Alexander V. Stoyanov,
Pier Giorgio Righetti,
期刊:
ELECTROPHORESIS
(WILEY Available online 1997)
卷期:
Volume 18,
issue 3‐4
页码: 344-348
ISSN:0173-0835
年代: 1997
DOI:10.1002/elps.1150180308
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Immobilized pH gradients;Isoelectric focusing;Ion adsorption;Desorption
数据来源: WILEY
摘要:
AbstractA simple theoretical model of low molecular mass ion adsorption on a weak exchanger is proposed. For a system containing immobilized charges in uniform concentration the pH change values connected with neutral salt addition and followed by further washing and voltage application are evaluated. The question of stability of the moving boundary, arising in the area near the electrodes, is considered for the constant current stabilization at different values of Immobiline concentration, pKvalues of Immoboline and salt concentration. The threshold values of ion mobility capable of providing a sharp salt front formation are also evaluated for conditions of strong adsorption. We found that, with extremely acidic Immobilines, a sharp salt front does not arise under the latter condition, even in the case of very high Immobiline concentrations.
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