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Thermometric Determination of Michaelis Constants

 

作者: Kathryn Barclay,   Neil Jespersen,  

 

期刊: Analytical Letters  （Taylor Available online 1975）
卷期: Volume 8, issue 1  

页码: 33-40

 

ISSN:0003-2719

 

年代: 1975

 

DOI:10.1080/00032717508060358

 

出版商: Taylor & Francis Group

 

关键词: thermometrlc;lactate dehydrogenase;Michaelis constants

 

数据来源: Taylor

 

摘要:

Thermometrlc detection methods have been applied to the enzymatic reduction of pyruvate to lactate. “Kinetic curves” obtained have been used to generate data for Lineweaver-Burk plots in order to evaluate the Michaelis constant of pyruvate. Results show that reasonably good values are obtained and that the well known substrate inhibition feature of this process is also observed.

 

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