A Novel Enzyme Membrane Electrode for Oxalate Determination in Foodstuffs
作者:
C.H. Assolant-vinet,
G. Bardeletti,
P.R. Coulet,
期刊:
Analytical Letters
(Taylor Available online 1987)
卷期:
Volume 20,
issue 4
页码: 513-527
ISSN:0003-2719
年代: 1987
DOI:10.1080/00032718708067985
出版商: Taylor & Francis Group
关键词: Key-words: Immobilized Enzyme, Oxalate, Biosensors, Preactivated Membrane, Foodstuffs
数据来源: Taylor
摘要:
An enzyme membrane electrode usable for the assay of oxalate in foodstuffs is described. A commercially available preactivated polyamide membrane was used for the immobilization of oxalate oxidase. The bioactive disk thus obtained was associated with an amperometric transducer. The resulting self-contained enzyme electrode wich allows oxalate determination in various materials with minimal pretreatment exhibits a linear calibration ranging from 10–7 M and 10–4 M in the cell. The response-time was comprised between 20 seconds and 1 minute, depending on the oxalate content in the sample. The electrode-response was very stable for at least 4 months, a period during which more than 150 assays were performed.
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