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A Novel Enzyme Membrane Electrode for Oxalate Determination in Foodstuffs

 

作者: C.H. Assolant-vinet,   G. Bardeletti,   P.R. Coulet,  

 

期刊: Analytical Letters  (Taylor Available online 1987)
卷期: Volume 20, issue 4  

页码: 513-527

 

ISSN:0003-2719

 

年代: 1987

 

DOI:10.1080/00032718708067985

 

出版商: Taylor & Francis Group

 

关键词: Key-words: Immobilized Enzyme, Oxalate, Biosensors, Preactivated Membrane, Foodstuffs

 

数据来源: Taylor

 

摘要:

An enzyme membrane electrode usable for the assay of oxalate in foodstuffs is described. A commercially available preactivated polyamide membrane was used for the immobilization of oxalate oxidase. The bioactive disk thus obtained was associated with an amperometric transducer. The resulting self-contained enzyme electrode wich allows oxalate determination in various materials with minimal pretreatment exhibits a linear calibration ranging from 10–7 M and 10–4 M in the cell. The response-time was comprised between 20 seconds and 1 minute, depending on the oxalate content in the sample. The electrode-response was very stable for at least 4 months, a period during which more than 150 assays were performed.

 

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