&NA;The ryanodine receptor (RyR) in aortic and vas deferens smooth muscle was localized using immunofluorescence confocal microscopy and immunoelectron microscopy. Indirect immunofluorescent labeling of aortic smooth muscle with anti‐RyR antibodies showed a patchy network‐like staining pattern throughout the cell cytoplasm, excluding nuclei, in aortic smooth muscle and localized predominantly to the cell periphery in the vas deferens. This distribution is consistent with that of the sarcoplasmic reticulum (SR) network, as demonstrated by electron micrographs of osmium ferrocyanide‐stained SR in the two smooth muscles. Immunoelectron microscopy of vas deferens smooth muscle showed anti‐RyR antibodies localized to both the sparse central and predominant peripheral SR elements. We conclude that RyR‐Ca2+‐releasechannels are present in both the peripheral and central SR in aortic and vas deferens smooth muscle. This distribution is consistent with the possibility that both regions are release sites, as indicated by results of electron probe analysis, which show a decrease in the Ca2+content of both peripheral and internal SR in stimulated smooth muscles. The complex distribution of inositol 1,4,5‐trisphosphate and ryanodine receptors (present study) is compatible with their proposed roles as agonist‐induced Ca2+‐releasechannels and origins of Ca2+sparks, Ca2+oscillations, and Ca2+waves. (Circ Res. 1998;82:175‐185.)