AbstractNectar sugar composition, nectar secretion patterns, and effects of removal are compared in a natural hybrid experimentally supported (Lycium ciliatum × cestroides) and its parental species (L. ciliatumandL. cestroides). The nectar ofL. ciliatumandL. ciliatum×cestroidesis hexose dominant, but each taxon shows a distinctive pattern: more glucose than fructose in the former and the opposite pattern in the latter.L. cestroideshas sucrose‐rich nectar with more fructose than glucose. Both parental species show a similar sugar production pattern: the sugar content declines at the end of the flower lifetime due to nectar reabsorption. In contrast, in the hybrid, nectar secretion is continuous and there is no nectar reabsorption. Data obtained among the taxa are correlated with corolla depth: the deeper the corolla tube, the more diluted and the higher the volume of the nectar. Nectar production after removal is increased inL. cestroideswhereas it is not affected in the hybrid or inL. ciliatum. Chemical composition and response to removal relate the hybrid to the maternal parent (L. ciliat