Interaction of poly(sodium vinyl sulfonate) and its surface graft with antithrombin III
作者:
Yoshihiro Ito,
Lin‐Shu Liu,
Yukio Imanishi,
期刊:
Journal of Biomedical Materials Research
(WILEY Available online 1991)
卷期:
Volume 25,
issue 1
页码: 99-115
ISSN:0021-9304
年代: 1991
DOI:10.1002/jbm.820250108
出版商: John Wiley&Sons, Inc.
数据来源: WILEY
摘要:
AbstractPoly(sodium vinyl sulfonate) (PVS) was found to be 1/14 times as active as heparin in inducing the conformational change and activation of antithrombin III. The conformational change of antithrombin III was investigated in terms of the intrinsic fluorescence of tryptophan residue, the extrinsic fluorescence using 1,6‐diphenyl‐1,3,5‐hexatriene as fluorescent probe, and Fourier‐transform infrared spectroscopy. It was evident in the experiment using 2,4,6‐trinitrobenzene sulfonate that PVS elicited the activity of antithrombin III by interacting with amino groups of the protein as does heparin.Sodium vinyl sulfonate was graft‐polymerized onto polyetherurethane (PEU) film that was treated with glow discharge in advance. PVS‐grafted PEU film adsorbed antithrombin III easily and, like ungrafted PVS, induced conformational change and activation of antithrombin III. However, the mechanism of interaction of the PVS graft with antithrombin III did not seem to be completely the same as that of ungrafted PV
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