Twenty products associated with the metabolism of zearalenone by Fusarium roseum were analyzed with respect to time and culture conditions. By statistical analysis a set of six metabolites possibly related to zearalenone were selected. These products were characterized by gas chromatography‐mass spectrometry and other methods. The chemical structures of these six confirmed the statistical relationships, and evidence for the formation of these materials from zearalenone is presented. A suggested scheme of metabolism of zearalenone by F. roseum includes the formation of the two isomers of 8'‐hydroxyzearalenone, 6’,8'‐dihydroxyzearalene, 6‐(carboxypentyl)‐β‐resorcyclic acid, and phenylacetic acid derivatives.