Quantitation of Transition Metals Using Genetically Engineered Enzymes Carrying Polyhistidine Tails
作者:
Magnus Olin,
Jonas Carlsson,
Leif Bülow,
期刊:
Analytical Letters
(Taylor Available online 1995)
卷期:
Volume 28,
issue 7
页码: 1159-1171
ISSN:0003-2719
年代: 1995
DOI:10.1080/00032719508000335
出版商: Taylor & Francis Group
关键词: Metal analysis;histidine modified enzymes;lactate dehydrogenase;galactose dehydrogenase;immobilized metal affinity chromatography
数据来源: Taylor
摘要:
Two genetically modified enzymes, galactose dehydrogenase and lactate dehydrogenase carrying polyhistidine tails, were used to develop a simple method for quantitation of transition metals. The samples containing copper, nickel or zinc were initially adsorbed to Chelating Sepharose. Excess of engineered enzyme was added and allowed to bind to the chelated metal ions via their additional histidine tails. The amount of metal ions could be determined by addition of enzyme substrate. The detection limits for copper and zinc were 0.01 μmole and 0.2 μmole, respectively.
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