Induction of Immediate Early and Stress Genes in Rat Proximal Tubule Epithelium Following Injury: The Significance of Cytosolic Ionized Calcium
作者:
YamamotoNaritaka,
MakiAtsuhiko,
SwannJohn D.,
BerezeskyIrene K.,
TrumpBenjamin F.,
期刊:
Renal Failure
(Taylor Available online 1993)
卷期:
Volume 15,
issue 2
页码: 163-171
ISSN:0886-022X
年代: 1993
DOI:10.3109/08860229309046148
出版商: Taylor&Francis
数据来源: Taylor
摘要:
This study was designed to investigate the influence of intracellular ionized calcium ([Ca2+]i) on the induction of c-fos, c-jun, c-myc, and hsp70 genes after ox-idant stress induced by xanthinelxanthine oxidase (X/XOD) treatment or after heat shock using primary cultures of rat proximal tubule epithelium (PTE). X/XOD (500μM/25 mU/mL) induced all of these genes; ionomycin also resulted in similar kinetics of induction of all genes. The expression of both c-fos following X/XOD treatment and hsp70 following heat shock was markedly decreased through che-lation of [Ca2+]iby Quin 21AM. The c-fos expression following X/XOD treatment was partly reduced by a protein kinase C inhibitor, staurosporine (ST), and markedly inhibited by another protein kinase inhibitor, 2-aminopurine (2AP), while both ST and 2AP markedly reduced hsp70 expression. The ADP-ribosylation transferase inhibitor 3-aminobenzamide had on effect on either c-fos or hsp70 expression. These results suggest that cell injuries leading to increased [Ca2+]iin PTE result in induction of c-fos, c-jun, c-myc, and hsp70; and that the activation of c-fos and hsp70 genes may be regulated by [Ca2+]iand [Ca2+]i-dependent protein kinases.
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