Function of mevalonate in tobacco cell proliferation
作者:
Andréa Hemmerlin,
SpencerC. Brown,
ThomasJ. Bach,
期刊:
Acta Botanica Gallica
(Taylor Available online 1999)
卷期:
Volume 146,
issue 1
页码: 85-100
ISSN:1253-8078
年代: 1999
DOI:10.1080/12538078.1999.10515804
出版商: Taylor & Francis Group
关键词: mevalonate;mevinolin (lovastatin);tobacco BY-2 cells;cell cycle regulation;cell death;protein isoprenylation;mévalonate;mévinoline (lovastatine);cellules BY-2 de Tabac;régulation du cycle cellulaire;mort cellulaire;isoprénylation des p
数据来源: Taylor
摘要:
We have used mevinolin, an inhibitor of 3-hydroxy-3-methylglutaryl- CoA reductase, to study the importance of mevalonic acid (MVA) for cell cycle progression of tobacco (Nicotiana tabacumL.) BY-2 (TBY-2) cells. Previously we have shown that in principal mevinolin blocks cells mainly at the end of G1 phase, but that cells are sensitive towards the inhibitor at the end of mitosis/entry G1 phase (Hemmerlin & Bach, 1998). The mevinolin—induced cell-cycle arrest was reversed by addition of exogenous MVA. It is shown that blockage of MVA synthesis might lead to variations in intracellular pH, which could be involved in the regulation of cell proliferation. SDS—PAGE analyses of total proteins and GTP—blot analyses indicate mevinolin—induced changes in the pattern, which can be reversed by addition of MVA. In preliminary experiments it has been demonstrated that artificial increase of the cytosolic pH partially released mevinolin—treated cells from the arrest in late G1 phase. A model is presented of how such proteins and pH variations could interact with cell cycle regulation.
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