Comparative assessment of herbicide phytotoxicity toSelenastrum capricornutumusing microplate and flask bioassay procedures
作者:
D. St‐Laurent,
C. Blaise,
P. Macquarrie,
R. Scroggins,
B. Trottier,
期刊:
Environmental Toxicology and Water Quality
(WILEY Available online 1992)
卷期:
Volume 7,
issue 1
页码: 35-48
ISSN:1053-4725
年代: 1992
DOI:10.1002/tox.2530070104
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractFour reference toxicants (Cr6+, Cu2+, Zn2+, phenol) and 9 herbicides (imazamethabenz, 2,4‐dichlorophenoxyacetic acid, picloram, glyphosate, bromoxynil, metolachlor, diquat dibromide, hexazinone, cyanazine) were appraised using both the microplate and flask assay Ninety‐six hour EC50s determined withSelenastrum capricornutumas the indicator species essentially demonstrated good intermethodological data concordance for all chemicals, with the exception of diquat dibromide, whose phytotoxicity in the microplate assay (EC50= 4.9 μg · L−1) was nearly 7 times that of the flask assay (EC50= 34.2 μg · L−1). Comparisons with other data in the scientific literature relating to similar herbicides with the same or different green algal indicator species appeared to corroborate the overall data obtained in our study. More than 4 orders of magnitude separated the most toxic (cyanazine, flask, and microplate EC50s of 17.6 and 16.9 μg · L−1, respectively) and the least toxic (imazamethabenz, flask, and microplate EC50s of 89.1 and 91.1 mg · L−1, respectively) herbicides. The biprocedural phytotoxicity comparison described in this work suggests that the simpler algal microplate assay can be an appropriate alternative to the flask technique to evaluate the algal growth inhibition eff
点击下载:
PDF
(755KB)
返 回