The role of pili in the attachment ofPseudomonas aeruginosato unworn hydrogel contact lenses
作者:
FletcherErica L.,
WeissmanBarry A.,
EfronNathan,
FleiszigSuzanne M. J.,
CurcioAnita J.,
BrennanNoel A.,
期刊:
Current Eye Research
(Taylor Available online 1993)
卷期:
Volume 12,
issue 12
页码: 1067-1071
ISSN:0271-3683
年代: 1993
DOI:10.3109/02713689309033504
出版商: Taylor&Francis
关键词: Pseudomonas aeruginosa;cornea;contact lens;infective keratitis;bacterial adherence;pili
数据来源: Taylor
摘要:
Contamination of contact lenses is thought to increase the risk of infectious keratins, yet factors promoting attachment of bacteria to contact lenses are not fully understood. It has been suggested that strains ofPseudomonas aeruginosaattach to mucosal surfaces via pili which are appendages found on some strains. This study investigated the role of pili and the effect of incubation time on the attachment ofP. aeruginosato 20 unworn hydrogel lenses representative of each of the four FDA categories. Ten lenses were incubated for 15 minutes and another ten for 180 minutes. Lenses were incubated with either PAK+P. aeruginosawhich possessed pili or its isogenic mutant pair, PAK-, which was genetically similar except for the absence of pili. Bacteria were quantified, following homogenization of the contact lens, by viable counts. Non-piliated bacteria were significantly more likely to adhere to the lenses (p<0.001). A significant interaction between lens type and incubation time was observed (p<0.05); thus it is difficult to generalize about either of these effects in isolation. These results show that surface characteristics may confer an attachment advantage to bacteria.
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