Mass–length ratio of fibrin fibers from gel permeation and light scattering
作者:
Marcus E. Carr,
Linus L. Shen,
Jan Hermans,
期刊:
Biopolymers
(WILEY Available online 1977)
卷期:
Volume 16,
issue 1
页码: 1-15
ISSN:0006-3525
年代: 1977
DOI:10.1002/bip.1977.360160102
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractMass–length ratios of fibers in fibrin gels were obtained from measurements of the angular dependence of the intensity of light scattered by dilute gels and from the permeability of more concentrated gels. The permeability was determined by measuring the forced flow of buffer through a short column of gel held in a glass tube. The results obtained with the two methods are consistent. At high pH and high ionic strength the mass–length ratio is found to be that calculated for a protofibril, i.e., a double strand of fibrin molecules laid end to end at a separation of 450 Å. This same value is found under conditions where the polymerized fibrin is not gelled (pH 10.25) and where it is just gelled (pH 10.0). At pH 7.4, ionic strength 0.35, the fibers are found to have a higher mass–length ratio, the average fiber consisting of some three protofibrils associated laterally. At pH 7.4 and low ionic strength (0.10M) the fibers are up to a hundred times more m
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